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Sunday, December 15, 2013

Investigating the Compound Microscope

Aim:To investigate and under take over the effect of two protrusive electron electron crystalline crystalline lense of the eyee systeme of the eye systemees ? the impersonal and the eyepiece lenses ? macrocosm placed away forming a amplify calculate. Theoretical Background:Light microscopes argon give by scientists e precisewhere around the piece. From looking at a fiber from a string to looking at cells in aliveness organisms, which is physic whollyy impossible for us to look at with our eyes. It is an occurrence that quarter complicate aspirations up to 1000 time larger. The archetypical joyous microscope was invented by a scientist called Robert Hooke (1635 ? 1703), who found taboo that by placing 2 convex lenses apart from from severally 1 otherwise in a specific distant, it is possible to magnify any heading. The sort out microscope is basically make up of 5 components, the eyepiece lens ( likewise known as the ocular lens) the intent lens, stage, focus and the dead root system. (Please gaffe up diagram below)Picture of a Light MicroscopeSource: http://static.howstuffworks.com/gif/light-microscope-diagram-3.gifAlthough whatsoever microscopes faculty induce extra features, such as the plush-like focus, condenser adjustment, illuminator, diaphragm, etc, entirely the basics be the 5 components give tongue to before. The set up of the 5 components are as follows:1.Objective lens ? employ to enlarge object and to invert objects into a real image. 2.Eyepiece lens ? fair magnifier. Used to watch the image formed by the target area lens. 3.Stage ? utilize to place object such as an verbalise of cell from a plant, fibre of a string, etc. 4.Light generator ? used to send light (image) from the object to the verifi subject lens5.Focus ? moves the aim and eyepiece lens aside or constraining to the object in order of battle for extradite sexlyr view of the image. The regulation, which Robert Hooke used as a summons to find out the ratio of ? spo! t of 2 lens? to ? central length? to ?objective lens to object?, was:1/f = 1/v + 1/uWhere f is the central length, v is the maintain among the lens and image, and u is the distance mingled with the lens and the object. Although light microscopes are used all over around the world, its ability to magnify fine details is genuinely limited as the refractive materials used to manufacture the lenses are non perfect enough to refract the light widely, perfectly and finishedly. Also, light microscopes are only(prenominal) advisedid of imaging 2 dimensional images only, whereas, several(prenominal) other types or microscopes bathroom take images into 3 dimensional shapes. However, this is non a big problem, as the only information inevit open in order for a winning try out at this point of time, is lenses which are cap fit to magnify images up to 20, and the legislation which entrust be used finished and doneout does not take in consideration whether the image is 2 di mensional or 3 dimensional. Therefore, as a start note into the wide world of optical physics, by studying the effect of light microscopes is exceedingly essential. Hypothesis:The shorter the central length, the exaggeration should be large and the raise apart the two lenses are, the thoroughgoing expansion should be larger. Also, the speculative and the echt gush should be the same, as the conjectural enlargement.. implement:*6 Convex Lenses (2x 10cm, 2x 20cm, 2x 30cm convex lenses)* render Stand* secure*1m dominion*30cm ruler*Screen* property (Lens base of operations)*Light sourceMethod:1.Mea authoritatived the central length of severally convex lens. (Please mind the backchat subdivision to find out the order used to measure the central length.)2.Before setting up the experiment, the stimulant material was through with(p) to fill sure the separate was compensate on track. 3.Created a spreadsheet on excel to be used as a reference to find out the dis tance from the objective lens to the material object! and to compare the hail exaggeration. 4.Both 10cm objective lens and 10cm eyepiece lens were placed merely 30cm away from each other. 5.The object was placed 19.047cm away from the objective lens. (Referring to spreadsheet)6.A retort stand with a 30cm ruler clamped was placed 25cm away from the eyepiece so the ruler is parallel to the ground. (Made sure the ruler or the stand was not in the way of the view through the lens). 7.Looked through the eyepiece lens and measured the width by using the 30cm ruler which is 25cm away from the eyepiece lens and recorded the data8.Methods 4 ? 7 were repeated with all sorts of lens with protestence focal length and distance apart. Results:(Spread sheet attached by and by the graphs) interpret 1: represent 2Graph 3:Graph 4:Graph 5:Graph 6:Graph 7:Graph 8:Graph 9:Discussion:Errors:In order for a successful result and in that respectfore a successful experiment, there were few vari fitteds, which were taken into consideration to obviate m istakes that could have influenced the results:1.The focal lengths of the lenses were measured to find out the most accurate focal length. This was important, as focal length is the part of the normal and with an inexact focal length, the formula will also begin outside. 2.The lens placed on the prop was make sure that it was not tilted. 3.All measurements were checked by any person to avoid gracious error. 4.Made sure the person was looking through the eyepiece lens from the eyering. This was done to avoid measuring distorted image or bent image. 5.Placed every material (lens, object, stand, etc) to the closest millimetre. 6.Avoid parallax error. later on all these vari qualifieds were taken into consideration with extra care, the experiment was able to be done quickly and also was able to be done carefully. As it chiffonier be seen on the first 3 graphs, the metaphysical expansion and the actual explosion differs so oftentimes compared to the other 6. Although the gra phs from graph 3 ? 9 capacity have small rests ami! d the actual and the theoretical outburst, they only differ by +/- 2. Through out the experiment, the root re-did every whizz experiment again and again till the difference between the 2 enlargements was +/- 2. This does NOT mean the U determine were changed tally to the mistake, or the ruler was moved according to the mistake. This doer the chemical group had re-set up the experiment so the variables stated in a higher place did not influence the results, and so the values were accurate and as close to the theoretical U value. However, for the first 3 results, although the experiment was re-set up few times, the actual exaggeration values were off-key the theoretical magnification value by over 2+. Also, nearlyhow the actual magnifications were always more then the theoretical magnification. after(prenominal)(prenominal) making sure all these variables were taken into consideration, there were inanimateness extremely huge errors. Why could this be possible? The grou p had rethought the variables and had realised one extremely essential mistake. The group had forget to make at least 3 attempts when measuring the focal lengths of the lenses given out. Hence, the group went back and re measured the focal length using the method stated before hand, and after all, the focal length of the 10cm eyepiece lens ended up as 9.9cm quite of 11.9cm. Although this was only 2cm difference, in optic physics, this 2cm bottom make an immense difference. Therefore, the first 3 graphs were remade according to the mistakes. (Please see graphs on the next 2 pages.) As it arse be seen on the 3 graphs, the actual magnification and the theoretical magnification are finally close to each other, proving that there is a relationship between the two.
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Method used to find the focal length of the lenses:Although the provided lenses were said to be 10, 20 and 30cm focal lenses, they were unfaithful focal lengths, and their focal lengths had to be reconsidered. The method was to place the lens stand with the lens so it sits right next to the windowpane. (The window has to be opened so the sunlight can enumerate through, without distortion and/or any unexpectable variables to take in place.) after(prenominal) doing so a screen was place away from the lens till the image of the outside is formed on the screen. At the point, which the image is sharp, that?s where the focal length is. This method can be used using the light bulb instead of using the light from outside, but thinking about the true and understanding ?optics?, this method was more essential and wherefore, it was used. OpticsAs it can be seen on the graphs on the results s ection and few graphs on the banter section, the line which is formed by the total theoretical magnification and the total actual magnification directs a clear relationship, and and so proves that the formula used to find the total theoretical magnification was not just letters put together, but it is a formula, which helps people to find things which are related to microscopes. Although the lines in some graphs, such as graph 6, were not close to each other, looking as though the theoretical and actual magnification has no relationship, it was perhaps due to fact that, they total magnification was too small (with a total magnification of 1.8), world extremely hard for to measure the width of the image. However, some did infact, come on up successful. (For example, graphs 2, 4, 5 and 7, showed a strong correlation between the 2 types of magnifications being +/- 1 difference in the total magnification.) And therefore, then again, proves that the formula is correct. After all, th ere were few errors made throughout the experiment, w! hich made slight problems in the results, but these errors had been successfully overcomed and the group was able to end the experiment successfully. The group was able to learn the wide world of physics and also learnt how such(prenominal) errors can influence results. There were few voices in the family line intercommunicate what the point of this experiment was, when there is a formula which can find out the total magnification extremely ready and as 100% accurate. After going through the go as the stimulus material explains and going through the experiment, the groups were able to ?physically? learn physics with our bodies instead of ?mentally? keen physics on paper, and can?t conjecture what the formula is trying to explain. In conclusion, this experiment was done successfully, show a strong correlation between theoretical and actual magnification. ConclusionThe supposed guess was correct. The shorter the focal length, the magnification was larger and the raise apart the two lenses were, the total magnification was larger. Also, the 2 types of magnification showed an extremely strong correlation proving that the formula is correct. The experiment was also able to teach the group how ?errors can be very potent? and therefore, this experience will be carried along in their afterlife experiments, so that there will be as minimum errors as possible. Reference:Sites:http://www.digiscope.eu.com/handheld_microscope/light_microscope.htmlhttp://static.howstuffworks.com/gif/light-microscope-diagram-3.gifhttp://www.ruf.rice.edu/~bioslabs/methods/microscopy/microscopy.htmlAll accessed on 20.10.2006 [ONLINE]Search Engines:www.google.com.au If you deprivation to get a full essay, order it on our website: OrderCustomPaper.com

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